Sentence examples for stringent negative from inspiring English sources
Additionally, multiple stringent negative enrichments using targets to which the antibody fragment should not bind were selected against.
These results indicate that the genomes of subterranean lineages evolved under less stringent negative selection compared with their terrestrial relatives.
The library screening and enrichment strategy used in this work focused on multiple, stringent negative enrichment targets to identify a scFv specific for androgen-dependent prostate cancer cells.
This stringent negative selection scheme was utilized to obtain two other ssDNA MREs that bind to their respective targets with high affinity and specificity [ 15, 16].
For instance, any study that uses SMLM for quantitative analysis must have stringent negative and positive controls, since artifacts in the imaging or analysis methods can give rise to wrong inferences.
These results revealed that, prevalent GO terms (731) were related to the higher dN/dS ratios in subterranean lineages than in their terrestrial relatives, which indicates that the higher dN/dS ratios in subterranean lineages was not influenced by adaptive evolution of some particular functions, but more likely by the less stringent negative selection that impacts the whole genome.
Consistent with the result that both pre-treated LB medium and LB medium with applied current caused similar reduction of cell viability (Figure 2), the genes under negative stringent control were not significantly repressed.
Expression of these genes in relA/spoT mutant was significantly higher than in wild-type strain and rpoN mutant, which could suggest that this operon is regulated by the stringent response in negative manner.
However, such actions are likely to result in less stringent regulations and other negative welfare effects.
Particularly stringent evaluation of simultaneous negative control experiments is required to identify experimental artefact within this region.
The stringent method includes additional negative gating steps, mainly for erythroid lineage-specific cell surface protein markers (Additional file 1: Figure S1; Additional file 1: Table S1).
