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However, using the more stringent matrix similarity cut-off value of 0.98, the fold occurrences of the AhR sites in the promoters of all Aroclor 1254-regulated genes including genes involved in apoptosis is clearly increased as compared to promoters of unregulated genes.
In a similar way as the previously described amino acid framework, our character trimming method is more accurate when using a stringent matrix (e.g. PAM-1) with closely related DNA sequences, and when using a relaxed matrix (e.g. PAM-250) with distantly related ones.
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Negative controls consisting of housekeeping genes (GAPDH, ACTB) and randomized DNA sequences were used to evaluate and eliminate less stringent matrices.
As shown in simulation results (see below), our entropy-based character trimming method performs better when using stringent matrices (e.g. BLOSUM95) for closely related sequences, and, reciprocally, when using more relaxed matrices (e.g. BLOSUM30) for distantly related sequences.
To the minor exception of Noisy with ML trees, BMGE trimming with the (less stringent) BLOSUM30 matrix allows reconstructing the significantly best trees with level of divergence ×3.
However, BMGE with the stringent BLOSUM95 similarity matrix leads to a different ML phylogenetic tree (right-hand tree in Figure 6).
If even the less stringent trimming procedure engendered a matrix with fewer than 4 columns, the matrix was discarded.
As the value of x grows, the clustering criterion becomes more stringent and the resulted matrices set grows, and vice versa.
The study outcomes were able to overcome the limitations of stringent laboratory preparation of calibration matrices by extracting uncorrelated underlying factors in the data matrices through systematic application of PCA and factor analysis (FA).
The need for a more stringent solvent suggested that the biofilm matrix around the mutant is composed partly of a protein component.
Therefore we chose to use the PAM30 matrix which is fairly stringent and is also the default used by the NCBI BLAST server when dealing with peptide queries.
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