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Clearly, the choice of different specialized databases and perhaps less stringent match criteria has the potential of reducing this portion.
Because this approach compares short fragments of genomic DNA against ESTs, the use of stringent match conditions would fail to detect most BES containing intron-exon boundaries, whereas less stringent conditions would increase the number of false positives.
The GMAP alignment method, with stringent match criteria (using only high-quality reads, unique mappings, multiple-reads SNP support; details in Materials and Methods), produced a total of 10,778 predicted SNPs.
These sequences were processed so as to identify high-confidence tags; this processing included an initial mapping of the 3′ end (oligo-dT/dA-containing) tags to the current release of the M. truncatula genome using stringent match parameters, followed by a step that removed 3′ end tags that did not have a corresponding mapped 5′ paired end tag.
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Longfield argues the best way to tackle the problem of merry-go-round care placements is twofold: providing specialist training for foster carers – she highlights examples of such training in Ealing – and a more stringent matching process.
This comparison is based on stringent matching criteria to ensure that participants and non-participants were indeed closely comparable at least on dimensions we can observe (e.g. age, gender, tenure in the company and prior performance).
I then used MAST to search Drosophila proteomes for stringent matches (P < 0.00001 for each motif match).
Sensitivity analyses were conducted using a more stringent matching criterion on the propensity scores (0.001).
Notwithstanding stringent matching criteria for demographic and severity of illness data including mean blood glucose, we cannot completely exclude confounders.
Under the stringent matching criteria, it was found that the target numbers of both nematodes ranged from one to thousands.
However, because of the relatively stringent matching criteria, fewer than 2 controls per case were available in certain strata.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com