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We have included a less stringent group of subject genes, to allow for genes with slightly lower DNAm to be classified as subject; however, it is possible that some escape genes may be subjected to inactivation in a small proportion of cells.
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Twenty of these 36 singletons were previously included in one of the known clonal complexes, although with the stringent group definition of six of seven shared alleles their clonal relationship could not be established with this system.
The eBURST v3 software (available at http://www.mlst.net) was used to estimate the relationships among the isolates and to assign strains to a clonal complex (CC) using the stringent group definition of six of seven shared alleles.
The eBURST v3 software (available at http://www.mlst.net) was used to estimate the relationships among the isolates and to assign strains to a clonal complex (CC) using the stringent group definition of six of seven shared alleles [ 23].
More specifically, the "stringent" group was formed of both PNS_2 and PNS_H groups combined.
Because of our stringent group criteria, our sample size is small.
Therefore, 21 patients were left in the "stringent" group.
After stringent mapping and correction of suspicious data, the segregation data belonging to each wheat chromosome were pooled and re-analysed in Carthagene using less stringent grouping thresholds (rec freq = 0.3 LOD = 3).
In fact, on the question of embryo protection, the USA are more stringent than the group of advisers of the European Commission, because in the USA at least there is no public money for expensive embryo research.
These CNC-CNC interactions may in part explain their stringent conservation as a group of regulatory sequences.
In this study, the eBURST analysis was performed with the stringent (default) group definition of six out of seven shared alleles, as described at http://eburst.mlst.net/.net/
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