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Twenty of these 36 singletons were previously included in one of the known clonal complexes, although with the stringent group definition of six of seven shared alleles their clonal relationship could not be established with this system.
Therefore, 21 patients were left in the "stringent" group.
More specifically, the "stringent" group was formed of both PNS_2 and PNS_H groups combined.
Because of our stringent group criteria, our sample size is small.
The eBURST v3 software (available at http://www.mlst.net) was used to estimate the relationships among the isolates and to assign strains to a clonal complex (CC) using the stringent group definition of six of seven shared alleles.
The eBURST v3 software (available at http://www.mlst.net) was used to estimate the relationships among the isolates and to assign strains to a clonal complex (CC) using the stringent group definition of six of seven shared alleles [ 23].
Similar(52)
Using stringent grouping parameters (distance >20 cM and LOD value >8), we obtained 23 linkage groups.
Secondly, a smaller but more stringent grouping was made that included only women with lesions in a single region.
After stringent mapping and correction of suspicious data, the segregation data belonging to each wheat chromosome were pooled and re-analysed in Carthagene using less stringent grouping thresholds (rec freq = 0.3 LOD = 3).
Overall, the distribution of breed-wise data shows that implementing the stringent grouping strategy resulted in a clear separation (contrast) of wither height (stature) between the large and the small cohorts of the European and African categories.
The remaining isolates were assigned to the ST27 complex with a less-stringent group definition (Table), although ST101 (MNCM21) and ST104 (MNCM50) shared only 2 alleles with ST27 and were incorporated into this complex by a chaining effect.
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