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The stringent gate drive margin, instability issues due to high dv/dt and di/dt, cross talk due to parasitics, voltage overshoot and oscillations are analysed in detail using different gate drive circuits.
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The Gr-1+CD11b+ cells were sorted as MDSCs under stringent gating conditions (97 98% purity, BD FACSAriaTM cell sorter).
The script then output cell area, which after stringent gating, yielded approximately 1000 cell size measurements per condition.
As shown in Figure 2(d), these retinol+ HSC-KC doublets cannot be removed completely by density gradient but only by stringent gating in flow cytometric cell sorting).
Immune activation (CD38+\HLA DR+) and memory (CD45RA\CCR7) analyses were performed after stringent gating on singlet live (Aqua−) CD3+\CD4+ or CD3+\CD8+ T cells.
To accurately monitor HbF and HbA co-expression by flow cytometry, we firstly generated a stringent gating strategy developed using appropriate cellular controls (Fig 5).
By multi-parametric digital flow cytometry analysis, combined with a stringent gating algorithm (see the Materials and methods section for further details), we distinguished the viable singlet T cells from B/T-cell conjugates and determined their GFP content.
To exclude the possibility of detecting false-positive T cells expressing CD20, by analyzing T-cell/B-cell doublets, stringent gating was performed by using pulse height-versus-width parameter settings.
A sorting strategy that utilized the level of CD45 expression as a discriminator between pre-BI, pre-BII and naïve B-cells and a stringent gating strategy were employed to ensure that the 4 distinct populations of cells were collected with no overlap.
To sort out only viable T- or NK-singlet cells we employed a stringent multiparametric gating strategy.
The answer is for airlines to be a bit more stringent at the gate.
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