First, a subset of stringent CNVs found at a frequency <0.5% for the total sample set (including 676 ASD cases and 1000 PDx controls) was compiled.
In situations characterised by high responder frequencies such as stringent strain combinations and most likely in clinical transplantation we need powerful protocols for in vitro generation and differentiation of antigen specific regulatory T cells.
The stringent 100%-frequency may be unnecessary for other data sets as such distinction may not exist.
Marker sets with more stringent filtering showed a lower frequency of putative NCO GCs, which indicates that filtering reduces the incidence of false positives.
Reliable quantification of differentiation patterns, however, might require substantially more stringent minor allele frequency thresholds.
Second, we excluded any remaining transcripts with conserved protein coding potential, including the potential to produce small peptides, by performing stringent codon-substitution frequency (CSF) analysis (Lin et al., 2011).
In addition, given the high sequence error rate associated with 454 sequencing (approximately 0.5% [ 35]), we used stringent criteria (minimum allele frequency (MAF) ≥33%, coverage ≥10x) to avoid the selection of SNPs present at such low frequencies that they are likely to be the product of sequencing error.
The higher rates of depression found with NIMH-dAD criteria derived primarily from its less stringent requirements for the frequency and duration of symptoms.
Recently, 5G and IoT have sparked great research interest in developing the next generation front ends that can meet the more stringent requirements on performance, frequency scalability, bandwidth, power consumption, and spectral utilization efficiency.
As shown in Figure 3, it can be seen that for the least stringent energy threshold the frequency of a predicted miRNA target is greatest in transcripts-without-motif.
