Exact(16)
The results of this study showed that by using E. coli BL21 DE3) LOBSTR strain and in combination with our developed stringent expression and Ni-NTA purification protocols highly pure products in one purification step (>99% purity) can be obtained.
Hence, we prioritised our gene list using stringent expression criteria.
Larger values represent a higher propensity for CNV coupled with more stringent expression constraint (i.e., small Vg/Vm).
A more stringent expression level cutoff was placed where the discrepancy dramatically drops (dashed line at ln TPM) = −1.6).
Instead, a high abundance of transcription related genes could be responsible for the more stringent expression regulation.
Researchers who wish to increase the accuracy could apply more stringent expression level cutoff to the dataset.
Similar(44)
Even when applying a less stringent positive expression criterion (TPM ≥ 1), only 28.0% of rice genes expressed positive, providing compelling support for our method as also demonstrated with the previously determined p values for the enrichment of experimentally confirmed expression in our predicted gene set.
These stringent differential expression criteria were used henceforth.
Both of the corresponding wheat homologues of these genes were downregulated ~4-fold during senescence, but these differences were not significant according to our stringent differential expression criteria (Table 2).
Our estimation of positivity of breast cancers for our panel may have been conservative since we have been very stringent in calling expression positive, explaining why our rates of expression for GLUT1, CAIX, EGFR, MET, TfR, CAXII, and Mammaglobin are on the lower side compared to the literature [ 22, 34- 43].
But his own generation, Mr.Yu lamented, is more caught up in commercial pursuits and faces more stringent limits on expression.
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