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Thus, when making this more stringent estimation of protein numbers, we have classified 3,145 (59%) of the valid membrane proteins.
We also performed a more stringent estimation of the null distribution by randomly selecting 33 genes as the initial expression traits and replicating the analysis 1,000 times.
According to amino acid identity and phylogenetic analysis, MRTV was most closely related to IPPYV from the Central African Republic; however, the low bootstrap support precluded a stringent estimation of this closest relative.
Hence, we apply a second filter based on a more stringent estimation of copy number based on a BlastN search of the target genome with each consensus repeat as a query using the WU-Blast package.
Although the duplicated protein sequences were ~500 amino acid long on average, our use of the much longer sequences for simulation allows a very stringent estimation of the false positive rate.
Similar(55)
This method also allows objective estimation of cluster number, and stringent comparison between similar samples by co-clustering.
The findings indicate that the outdoor climatic conditions developed for cooling load estimation via the simulations are less stringent than the current outdoor design data and approaches adopted by local architectural and engineering practices.
Instead, variance components are usually estimated for a subset of the data, for which more stringent editing criteria are applied compared to data used for breeding value estimation.
It is clear that a stringent protocol for post-mortem examination is necessary for a truthful estimation of the epidemiology and incidence of invasive aspergillosis in ICU patients [ 6, 7].
These parameters are more stringent than previous SNP identification approaches [ 35] and provide a conservative estimation of variable sites in the coding regions of annotated transcripts.
Use of consensus InDels from two different bioinformatics packages followed by stringent filtration criteria provided confidence in the detected set and the FDR estimation suggested a high rate of validation.
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CEO of Professional Science Editing for Scientists @ prosciediting.com