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These approaches provide high temporal resolution and stringent discrimination between surface-connected and intracellular membranes.
We interpret these data as strong evidence that the PI-PLC treatment did not remove all external GPI-anchored protein, while reduction with MESNa provided more stringent discrimination between internal and external pools of the antibody.
Even so, this more stringent discrimination was not reflected in the discriminatory power as calculated by Simpson's index of diversity, which was 0.84 for the MOL-PCR method compared to 0.95 for the multiplex PCR-based method (Fang et al. 2012).
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Although this may not provide such stringent topological discrimination between intracellular vesicles and surface membrane as the sulfo-NHS-SS-biotin plus MESNa approach, there was a very high degree of co-localisation between FM1-43FX-positive puncta and transferrin.
The idea was to create a truly integrated community in suburbia, an idea that was surprisingly revolutionary for a state with some of the most stringent non-discrimination zoning laws in the nation.
Since the probes were selected from conserved regions within a family, the array was not designed for stringent species or strain discrimination.
Still, as the subgenre grows, its capacity for holding a mirror to today's problems ― climate change, stringent definitions of gender, and discrimination based on race or gender or nationality ― persists.
This suggests that the mechanism to maintain optimal color discrimination is more stringent in birds than in mammals.
However, at 8 months of age, the more stringent memory load resulted in discrimination of performance between the R26CT and R26CT-CRE mice.
In the literature, the majority of DC protection algorithms essentially use the first travelling waves initiated by a DC fault for fault discrimination due to the stringent time constraint in DC grid protection.
During his 11 months in office, at a time when the gay and lesbian community was encountering widespread hostility and discrimination, Milk passed a stringent gay rights ordinance for the city.
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