Sentence examples for stringent cycle from inspiring English sources

Thermocycling consisted of an initial stringent cycle (94°C for 3 minutes followed by 12 cycles of 94°C for 30 seconds, from 65 to 56°C decreasing by 0.70°C at each cycle for 45 seconds, 72°C for 120 seconds) and additional 25 cycles of 94°C for 30 seconds, 56°C for 45 seconds, 72°C for 90 120 seconds.

We attributed this increased cytotoxicity of NU1025/C-1305 combinatoon to a partial re-activation of wt p53 pathway in drug-treated HeLa cells and a more stringent cell cycle checkpoint control during G2/M transition that was associated with the enhanced cell death by mitotic catastrophe [48].

Notice that a more stringent 1% duty cycle limitation is applied to low-power wireless communications over ISM frequency bands.

The carbon corrosion and catalyst stability are also determined with more stringent corrosive test cycles, that is, maintaining the potential of the cell as high as 1.4 V for 180 s and later decreasing it to 0.6 V for 30 s versus reversible hydrogen electrode (RHE) for 500 cycles, mimicking the actual conditions of operating fuel cells.

A low annealing temperature (25°C) was used for the initial PCR amplification cycle; a stringent annealing temperature (55°C) was used thereafter to favor priming through the specific sequence (15 ).

We therefore took advantage of the dynamic aspect of PRICE quality filtering subjecting early cycles to very stringent quality requirements, followed by less stringent requirements in later cycles to fill in low-coverage regions (see Materials and Methods).

This feature also makes the materials requirements less stringent than for high temperature cycles, many of which require temperatures in the range of 800 900∘C.

The radioisotope-labeling PCR protocol used in this study is an advantageous method to obtain the specific PCR bands from a small amount of biopsy tissues under a stringent condition of minimal PCR cycles.

The antiviral activity of the extract was confirmed by a virus yield reduction assay, a more stringent test which allows multiple cycles of viral replication to occur before measuring the production of infectious viruses.

The patient is on his seventh cycle of therapy under stringent observation.

The advantages of this design include a shorter study duration, which minimizes the number of individuals who may drop out and also limits cycle-to-cycle variability while providing a more stringent evaluation of safety and tolerability.