To verify that the two separate networks were only partially related to the parameters we used for correlation, we tested less stringent combinations of Pearson's score and p-value (data not shown).
For the purpose of new biomarker identification, it is more suitable to use less stringent combinations, which results in higher recall.
Steve Jones, a dairy specialist based in the Forest of Dean, says that, instead of killing badgers, the solution to combating the disease is "a combination of stringent testing, controlled movements, vaccinations, quarantine and biosecurity".
This provides a robust and powerful dataset with low variance due to a combination of stringent experimental protocols and multiple biological replicates and its statistical strengths make possible sophisticated bioinformatic analyses and network predictions [55], [73].
This resulted in an average of around 15,000 probe sets analyzed per region Second, in order to control for type I errors, we also used a combination of stringent P-value thresholds (≤0.01 both at the ANOVA and post-hoc test), as well as a fold change of at least 1.3 in either direction.
Recently, we used a combination of stringent methods, that is, ribosome footprinting, next generation sequencing and advanced mass spectrometric technology, to discover a plethora of novel sORFs in cytomegalovirus, many of which we determined to exist at the protein level [ 23].
This assay allows for detection of microRNAs based on a combination of stringent hybridization and enzymatic primer extension on a microfluidic microarray starting from total RNA material, without the need for enrichment, amplification or labeling of the native RNA samples [ 9].
With a combination of increasingly stringent regulations, like energy codes for new buildings, as well as other conservation programs and rising prices, Chinese planners hope to preserve a similar low ratio of energy use to growth in the decade to come, Mr. Zhou said.
The differences in our study resulted from usage of the updated TAIR10.02 annotation and more stringent criteria; namely the exclusive combination of machine learning with sequence identity and consideration of the genomic context (e.g. synteny).
As the fraction hereof found by a stringent fracture definition using a combination of both ICD-10 and NOMESCO coding is 9,092/9.726 = 93.5%93.0-94.093.0-94.0%C.I)., the underestimation by this method is 6.5% (6.0-7.0%).
