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First, in previous studies of LAB phylogeny, less stringent clusters of orthologous groups (COGs) [ 6] and reciprocal best hits [ 8] methods were applied to identify putative orthologs.
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On the other hand, if (CC_{rg}(C,D_i)=0), it means that there is no common subtree between C and (D_i), therefore a stringent cluster contrast such as (CC_g(C,D_i)) (Proposition 0.4) will be 0 (Proposition 0.3).
A more stringent clustering analysis of the genes of clusters 11 and 12 resulted in four and three subgroups respectively (Fig. 3).
Keeping the E-value for constructing the BLAST similarity matrix constant, we observed the tendency that the larger data set required less stringent clustering parameters for the correct resolution of our gene families; that is, higher inflation values in case of TRIBE-MCL and lower E-value tree-cutoffs in case of MC-UPGMA (data not shown).
Stringent clustering and alignment parameters were used to limit the size of clusters for assembly.
Stringent clustering parameters were chosen to avoid problems with uncomplimentary probes in the probe design.
We selected parameters designed to produce a stringent clustering of the available sequences.
Three groups of clusters among the novel MITE-like (clusters 121-87; 137-144; and 43-197) were identified by the less stringent clustering.
For stringent clustering, peaks were selected that were rated for their ability to distinguish between different species.
PHRAP [ 52], under stringent clustering parameters (minimum score, 100; repeat stringency, 0.99), was used to assemble ESTs into contigs.
Initial assembly of ESTs into contigs used PHRAP [ 34], under stringent clustering parameters (minimum score: 100; repeat stringency: 0.99).
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