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Reduced diversity of samples analyzed at 44°C may rest on the fact that the primer is subjected to much more stringent bonding than that at the other annealing temperatures, therefore generating many sequences of a low number of species.
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Studies where the outcome is less critical to immediate health or life (e.g., to define research priorities) can afford a less stringent interpretation of consensus (Lindeman 1975; Bond & Bond 1982).
The fact that analytes induced the formation of new covalent bonds in aptazyme ligases (as opposed to noncovalent bonds in antibodies) potentiated stringent washing of the array, leading to improved signal-to-noise ratios and limits of detection.
As already mentioned, in CLIP-based protocols covalent bonds are induced through UV crosslinking, only the RNA sites with strong bonds are selected through stringent washes.
Recommended policy changes include a more stringent process for issuing permits for CAFOs, considering bonding for manure storage basins, limiting animal density per watershed, enhancing local control, and mandating environmental impact statements.
Activated caspases act through a catalytic Cys that hydrolyzes peptide bonds within the substrate, with a stringent specificity for Asp residue at P1 position (the nature of residues at positions P2, P3 and P4 depending on the caspase).
During the ensuing 30 years, more requirements were instituted to promote reclamation and some states had more stringent laws and regulations, and some had a reclamation bond.
It is shown that both adhesively bonded and bolted joints can be designed to fulfill stringent requirements, but it is clear that the former is the preferred alternative.
Berlin is pushing a more stringent approach in which old debt would be swapped into new 7-year bonds — something that credit agencies are more likely to interpret as a "credit event," or default.
Lloyds has also agreed not to make acquisitions for three to four years and will not be able to pay dividends to shareholders until the end of January 2012 because of stringent rules imposed by Brussels on the way it must treat owners of its bonds and other debt securities.
For bond funds, which don't make fat cash payouts, we're a little more stringent for Best Buys (see table).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com