For comparison with the potencies of other common arthritis therapies, a more stringent base-of-tail adjuvant-induced arthritis model was used [ 8].
In the recent 2006 NAAQS update, PM2.5 standards were made more stringent based on substantial scientific evidence of detrimental health effects (U.S. EPA 2006b).
Reads were mapped against the targeted contigs and then assembled within each cluster using MIRA software [ 91] and SNP detection was performed using stringent criteria based on base quality, absence of heterozygosity within genotypes and 2X minimal allele coverage.
Alignment of the sequences to their respective reference genome was performed with MAQ [ 18] using stringent conditions, based on high base quality values (maq assemble: - m = 2; - Q = 70 - q = 20), and using only sequences aligning to the reference genome with less than two mismatches.
Bearing these issues in mind, being stringent on base quality thresholds and only using contigs with highly significant BLAST hits (an e-value of ≤1e−4 is commonly used throughout the literature [ 22]) to previously published, putatively homologous sequences (e.g. those found in NCBI non-redundant and Swiss-Prot databases) will help to ensure high confidence in the resultant assembly.
In this manuscript, we devised more stringent criteria based on nucleotide conservation to extract highly conserved miRNA families.
More importantly, our stringent homology-based prediction means more than interspecies homology-based prediction.
More stringent guidelines, based on current best practice, could significantly improve rigor and consistency of toxicology and carcinogenicity assessments.
We could use techniques similar to the one we used for resolving NCBI and ENSEMBL conflicts, or develop more stringent criteria based on the authority concept.
The criteria for the ascertainment of tuberculosis cases in the present study were very stringent and based on clinical features (signs and symptoms of TB), chest X-ray findings, and positive sputum-smear tests.
