Sentence examples for stringent assays from inspiring English sources

As most stringent assays in murine systems chimaera formation and tetraploid complementation are commonly accepted.

As we need to develop at least some measure of pathogenicity of anti-dsDNA antibodies, we propose that initial anti-dsDNA antibody screening is done by sensitive ELISA and supplemented by more stringent assays.

Taken together, these results indicate that mRNA-derived iPSC clones from multiple independent fibroblast lines, with and without addition of VASA are fully reprogrammed by the most stringent assays available to date for human pluripotent cells (17).

For example in viral RNA detection, the "+strand" is the viral genome, while the "- strand" RNA is indicative of active viral replication; therefore, stringent assays were designed to specifically detect one strand versus the other [ 3- 6].

The stringent assays reported in this study increase the feasibility of exploring yet unknown cell death pathways in yeast.

ELISA screening (for lower avidity anti-dsDNA antibodies) followed by risk stratification through the use of more stringent assays (Farr, EliA, Crithidia) and restricting the relevance of anti-dsDNA antibody presence to specific clinical manifestations might provide us with a better index of the pathogenicity of anti-dsDNA antibodies.

In a more stringent assay, when treatment started after E. coli 5770 bacteraemia had already been established, three doses of 10 mg/kg A3-APO prolonged early survival at a rate similar to that of imipenem and reduced the bacterial counts to base level.

It is worth mentioning that the most stringent assay applied in the present study is the in vitro differentiation assay that corresponds to the teratoma-formation assay in vivo.

Setting stringent assay cutoffs to avoid detecting natural polymorphisms resulted in primer designs that provided sensitivities of 96% to >99% and specificities of >99% with samples that included highly polymorphic sequences (Table 2).

While the mice do not exhibit deficits on the accelerating rotarod, they do show deficits on the balance beam, a more stringent assay to detect gait disorders in mice.

This condition provides a stringent assay for downstream gene requirement.