Sentence examples for stringent alignment from inspiring English sources

An additional more stringent alignment was constructed by removing ambiguously aligned sites using Gblocks [ 69] as well as visual examination.

CQ-calculate uses stringent alignment criteria requiring the entire read to align with zero mismatches.

The process applied layers of filters to remove interfering non-miRNA sequences and provides stringent alignment for matching (zero and one mismatch data is separately aligned thereby allowing independent analysis and data mining of the two groups).

We applied the most robust and direct approach allowing the characterization of genome duplications that consists of aligning the available unigene set (31,798 elements) on itself using stringent alignment criteria and statistical validation described in Salse et al. [ 43].

A second strategy was to separately align the At and Dt genome reads from two varieties using very stringent alignment parameters or a precharacterized genome-specific SNP index to separate short reads into their subgenome of origin before varietal SNP identification (Byers et al. 2012; Salmon et al. 2012; Page et al. 2013a, b).

Of the 21 434 TACs, 16 622 (77.5%) could be mapped on to the soybean genome build 1.0.9 under fairly stringent alignment parameters.

In other words, ungapped alignments can be considered if the reference was generated from the same haploid sample as the assembly, and less stringent alignments can be considered if greater divergence is expected between the assembly and reference.

To study known sequence features involved in the recognition and distinction of splice sites, we performed large-scale but stringent alignments of cDNAs and ESTs to the human and mouse genome.

To answer this question, we have developed a strategy that combines very stringent alignments of the vertebrate sacsin domains with positional mapping of the human SACS mutations (for details, see Results).

To compare the types and levels of alternative tandem splice site exons occurring in different human organ systems and cell types, and to study known sequence features involved in the recognition and distinction of neighboring splice sites, we performed large-scale, stringent alignments of cDNA sequences and ESTs to the human and mouse genomes, followed by experimental validation.

Both of these properties facilitate the use of less-stringent alignment requirements than would be necessary with larger datasets, thereby lowering the amount of data required to ensure a successful assembly.