Exact(2)
False positives, if any, were sifted out in the next stage with a higher level of stringency where the results of BLAST, best reciprocal BLAST hit and HMM based search were compared and common hits satisfying the cutoff criteria were taken as confirmed hits of Ciona.
The procedure described herein identified 72 CNVRs with high stringency where the smallest CNVR was 38 kb.
Similar(58)
After hybridization, the slide was briefly (∼1 min) immersed in 2×phosphate-buffered saline (PBS) and then mounted in the multi-stringency array washer, where it was washed with the eight prepared and preheated buffers for 30 min at 37°C.
Altering the GO term distance threshold also had the anticipated result where the stringency of predictions was titrated as expected.
Although this hypothesis has yet to be experimentally proved, it is worth noting that cis-acting modules are much more prevalent at the end (receiver-output) of signalling pathways [ 14], where control stringency may be better achieved through direct domain domain allosteric control.
In all other genomes except D. persimilis where the high stringency search had identified one roo element, the low stringency search yielded additional roo and rooA copies.
These additional searches identified roo elements or remnants of roo elements in the genome of D. pseudoobscura, where the high stringency search had not found any elements.
After rinsing, the cartridge was placed in the NanoChip Reader where a specific thermal stringency was applied.
Adding a further stringency by analyzing the fold changes, where accepting a threshold >1 for the absolute log2-ratio reduced the list to 105 significant genes.
Although there seems little doubt that examiners do differ in their stringency, it is much less clear where those differences come from.
Their interaction partners outside the HLA region where included at the highest stringency level to visualise informative interaction pathways in which the HLA proteins are involved.
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