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Increasing our stringency thresholds for SNPs in the WGS decreased the number chip loci that were present in the list of SNPs identified by sequencing (n = 329,690; ~78%), but increased concordance to ~95%.
For fmgBC [ 47] and a site upstream of the dev operon [ 53] a ChIP-seq peak matched the position of MrpC2 binding in vitro, but the peak was not considered significantly enriched in one of the samples and therefore did not pass our stringency thresholds for inclusion among the 1608 high-confidence peaks (Table 2).
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The first two rows of table 3 compare the M1 and M2 model predictions under a low stringency threshold for missing data (site_p is set to 0.75).
Modules were grouped using a stringency threshold of 0.75.
The merged dynamic colors display the assigned changes when the stringency threshold of 0.75 was used.
The higher stringency threshold optimum for joint-family analysis compared with single-family analysis is congruent with a simulation of QTL-based selection (Blanc et al., 2008).
We measured the sensitivity, specificity and MCC at certain stringency thresholds.
To reduce false negative calls, we used stringency thresholds somewhat lower than those used in some other studies.
When applying certain stringency thresholds, it also significantly outperforms PSI-BLAST and BLAST at nearly all of the thresholds (Table 2).
Whether this difference is the result of the new implementation of Scansite3, potentially setting new underlying stringency thresholds, or due to the addition of a minimum stringency level is not clear.
In other words, using the trend for nonspecific genes at lower stringency levels, a stringency threshold was chosen that was high enough to consider 90% of the genes highly expressed in either fetal liver or placenta to be specifically derived from that tissue and not be a statistical artefact.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com