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Its governor, the peppery Leszek Balcerowicz, may be no loss to diplomacy, but he epitomises the radical economic reform and financial stringency that lies behind Poland's hearty economic growth.
On the other side of the coin, though, poaching inflicts significant costs on many universities, especially public institutions struggling with financial stringency, that lose researchers.
In addition, our ChIP-chip peak calls required consensus between three biological replicates, a stringency that may have eliminated true positives.
This p-value cut-off balances the requirement for stringency that will enrich for selection of true hits and lenience to ensure enough SNPs are included to allow a reasonably accurate measure of the Bayes Factor.
After the initial screening of probes, the next step is to select a combination of probe length, probe position and assay stringency that fulfill the criteria for assigning a genotype for each mutation site.
However, the latter authors identified a sequence as a roo element if it was only 50 bp long, a low stringency that is not appropriate for our analysis.
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To select genes from gene sets differentially expressed in EWS-FLI1 knockdown experiments (deltaKD) and in primary ESFT (deltaET) for intersection, we deliberately chose two cut-offs of different stringencies that would allow to maximize the number of genes for functional annotation in GS1 and GS2 at a reasonably significant p-value.
Utilizing the assembly algorithms incorporated in the SeqManII software (part of the DNASTAR suite from DNASTAR Inc ,Madison WI), the 9,159 ESTs were grouped into contigs and clusters by establishing assembly stringencies that generated groupings defined by the analysis protocols of McCarter et al., 2003 [ 34].
On the other hand, an RCT concerning physician behavior imposes stringencies that limit generalizability.
It has also been clearly shown, based on multiple hybridization stringencies, that this technique is highly stable and reproducible.
This suggests that the entire shared single copy genes list is not composed of a random set of genes, with 959 genes shared between Arabidopsis, Populus, Vitis and Oryza (APVO) as defined by the number of PlantTribes [ 63] at stringency 3.0 that contain a single member from each respective genome.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com