Sentence examples for stringency strategy from inspiring English sources

For nearly all assay strategies, a decrease in success rate was observed in the direction from methods A and B for assigning genotypes to methods C and D. This was especially clear for assay strategies with limited flexibility, e.g. one probe length or Tm-matched probe-set at one stringency, strategy 8 and 9, respectively.

For implementation of the second stringency control strategy, SPR spectrometer measuring flow cell with built-in high-precision temperature control and regulation as well as corresponding software was created.

As with Tm-matched probe-sets, processing microarrays at multiple stringencies resulted in higher success rates than when using a common stringency (compare strategy 5 and 8 in Figure 4).

The choice of stringency control strategies depends on various factors, including the sensing platform used.

In this study, we applied two stringency control strategies for surface plasmon resonance (SPR) detection of DNA hybridization and discrimination of completely and partially complementary 24-mer sequences.

We report the results of the application of two stringency control strategies for SPR detection of DNA hybridization and discrimination of completely and partially complementary oligonucleotides related to the human normal bcr and hybrid bcr-abl genes.

The aim of this research was to detect and discriminate the oligonucleotides related to the bcr-abl hybrid mRNA from those related to the normal bcr gene using two stringency control strategies based on the decrease in ionic strength of the hybridization buffer and the temperature elevation by SPR spectrometry biosensor.

To investigate this possibility, we developed a computer program (AnABlast) that generates profiles of accumulated alignments in query amino acid sequences using a low-stringency BLAST strategy.

However, because a high frequency of low significant alignments may reveal stretches of ancestral regions, we used a low-stringency BLAST strategy to include alignments beyond a significant similarity, a key step in our strategy to identify putative coding sequences with a biological and/or evolutionary significance (Fig. 1).

As test of the stringency of our strategy, we compared M. truncatula and G. max with each other and obtained a set of 15,219 orthologues as approximately expected with this strategy.

Amplification of glycosyl hydrolases was attempted by using a low stringency-degenerate primer PCR strategy, using total soil DNA and bulk DNA pooled from positive colonies as template.