Sentence examples for stringency solution from inspiring English sources

The next day, the membrane was washed twice with a high stringency solution (2X SSPE and 0.1% SDS), and twice with a low stringency solution (0.2X SSPE and 0.1% SDS) for 15 minutes each at room temperature.

Following hybridization, the glass slides were washed successively in a low stringency solution (1× SSC, 0.2%SDS) at 42°C for four minutes, high stringency solution (0.1× SSC, 0.2%SDS) at room temperature for four minutes, twice in 0.1× SSC for 2.5 minutes, and finally they were dipped in water, dried and stored in the dark until fluorescence was measured [ 91].

After washing in low stringency solution (2 × SSC, 0.1% wt/vol SDS), the signal was developed using the chemiluminescent BrightStart BioDetect kit (Ambion, Life Technologies, USA) and captured on Amersham Hyperfilm ECL (GE Healthcare, UK).

The membranes were hybridized in ExpressHyb™ solution (Clontech, Mountain View, CA, USA) at 68°C for 18 h with equivalent specific activity of P-labeled cDNA probes derived from 48A9 or ground control cells, followed by washing in a low stringency solution (2× SSC, 1% SDS; 3 times for 15 min) and then a high stringency solution (0.1× SSC, 0.5% SDS; 3 times for 15 min) at 68°C.

Membranes were then washed once with low-stringency solution (2 × SSC, 0.5% SDS), twice with high-stringency solution (0.2 × SSC, 0.5% SDS), each for 20 min at 68°C, and then exposed to BioMax MR film (Kodak) overnight.

The filters were washed twice in the pre-warmed (50 55°C) low stringency wash solution (2XSSC, 0.5% SDS, 0.1% Na pyrophosphate) for 15 min each.

Following hybridization, the samples were rinsed in 45 °C pre-warmed 1× SSC buffer for 5 min followed by 2 × 5 min washes in stringency wash solution (1 1 formamide: 1× SSC).

Filters were then washed four times for 15 min each at 22°C in high stringency wash solution (20 mM Na2HPO4, pH 7.2, 1% [w/v] SDS, and 1 mM EDTA), followed by three 15-min washes in the same solution at 68°C.

The oligoribonucleotides (IDT Technologies) were incubated at room temperature with equimolar amounts of the oligodeoxyribonucleotide in either in stringency wash solution (75 mM Tris-Cl, 112.5 mM NaCl) or in hybridization buffer (50% formamide/6 × SSPE), then the melting profile was performed in 1°C increments with constant monitoring at 260 nm.

The arrays were then washed extensively at 50°C under both low-and high-stringency conditions [2 × saline sodium citrate (SSC), 0.5% SDS for low-stringency wash solution, 0.5 × SSC, 0.5% SDS for high-stringency wash solution] for 2 × 30 min. The membranes were air dried, sealed in a clear plastic bag, and exposed to low-energy storage phosphoimage screens (Kodak, Rochester, NY).

The membrane was washed using the Ambion Northern Max Low Stringency and High Stringency wash solutions according to the manufacturer's instructions.