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The aim of this study is to measure the growth stringency requirement E. coli genes using antisense gene silencing, so as to evaluate degree of essentiality for cell viability.
As an additional stringency requirement, miRNAs were further considered only if they had at least a 2-fold difference in expression and were present in at least 75% of the samples in each group unless otherwise specified.
Comparisons of genes based on gene ontology (GO) to examine enrichments in the two datasets (all genes that were two-fold regulated by light and all genes whose light regulation met the q ≤ 0.2 stringency requirement) showed overall similar correspondences in GO terms for the top classes (Table S10), with unclassified genes missing in the q ≤ 0.2 set.
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Situations might arise where the need for fiscal flexibility and the fiscal stringency requirements will create a conflict and suboptimal macroeconomic policies are implemented.
In particular, we consider how fiscal stringency requirements like the Stability and Growth Pact affect fiscal policy design under EMU and study the consequences of the introduction of a fiscal transfer mechanism between countries.
The 'Excessive Deficits' procedure of the Maastricht treaty and its elaborations in the recent 'Stability and Growth Pact' introduce a set of fiscal stringency requirements on national fiscal policies.
To avoid double-counting interactions present in 2 databases from the same source compiled with different stringency requirements, only the most stringent PITA and picTar microRNA prediction files were used as inputs into the integration and filtering steps.
The -F option prevented blastall from using its complexity filter and the -f and -e options were our stringency requirements for keeping alignments.
Twenty seven potential Pfam domains were identified for 11 of the 117 predicted proteins (Table 2) while the other 106 sequences contained no known domains and, since these also have no BLAST matches against NR using the stringency requirements described here, they therefore represent novel proteins.
We chose to further analyse the genes obtained by the high-stringency requirements.
Therefore, RNA silencing using specific antisense sequences provides an approach to measure gene requirement stringency.
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