Sentence examples for stringency protocol from inspiring English sources

A high stringency protocol, suitable for systematic purification of polyclonal antibodies, is described.

FISH was performed largely following the high-stringency protocol of Vandekerckhove et al. (2002).

Based on the data from protocol evaluation, the high-stringency protocol was used to isolate methylated sequences from fcDNA samples.

Briefly, in the high-stringency protocol, methyl-binding protein (MBD2) was coupled to the beads following kit instructions; however, only 1 μL of beads per sample was used.

Prior to commencing experiments on fcDNA, we evaluated the performance of the methylation enrichment protocol with low (100 ng) DNA sample amounts, and developed a modified high-stringency protocol to limit the amount of non-specific DNA binding.

Amplification was performed in a thermal cycler (Appendorf) using a high stringency PCR protocol with high annealing temperature to ensure specificity of amplification.

Mutation of these products using a low-stringency PCR protocol followed by screening of large numbers of bacterial colonies allowed rapid creation of mutants with a variety of characteristics, including changes in color, maturation time, and brightness.

Although it is likely that some genuine SNPs may be overlooked, a high stringency SNPs calling protocol was utilized to avoid false SNPs calls.

It is likely that the stringency of our protocol accounts for the lower coverage and higher specificity of the set of hits retrieved; Modifications of the purification conditions could allow for the desired specificity.

We generated a total of 87 mini-libraries from the four major life cycle stages of honey bee workers (embryo, larva, pupa, adult) by the use of arbitrary primers and a low-stringency RT-PCR protocol [ 2].

Post hybridization washes followed this protocol: low stringency 4×15 minutes at 37°C, then high stringency 2×30 minutes at 55°C.