Sentence examples similar to stringency procedure from inspiring English sources

The substrate phosphorylated Maf1 was isolated by purification from a pph3Δ strain, using a 10 × His tag and a high-stringency procedure (see Materials and methods).

Mycelia subjected to this high-stringency selection procedure were used as starting material for further molecular analyses (see below).

The results of the GSEA analysis allow us to detect the regulation of gene clusters, including genes not detected in our first statistical analysis because of the stringency of the selection procedure.

However, the stringency of the filtering procedure can strongly affect downstream analyses and the final results.

This is probably due to a combination of the high conservation of the C and E domains used to design primers among all steroid nuclear receptors and the low stringency of touchdown PCR procedure that we used.

ASSIsT allows pre-selection of the stringency of the filtering procedure by customizing the following parameters: (1) Proportion of missing data, (2) Call Rate threshold, (3) Segregation distortion (χ P-value), (4) Frequency of not allowed genotypes (structured germplasm) and (5) Minor Allele Frequency.

Since the aim of our study is to assign function and phylogeny information to individual reads, rather than higher-stringency multi-read procedures like re-sequencing or assembly, we employed a minimum average Phred score of 10 as a cut-off filter in primary data-processing steps.

This approach is potentially problematic as false-negative reference standards could arise in patients in whom it is difficult to obtain sufficiently high quality sputum samples, in those with HIV-associated TB who do not have pulmonary involvement and also due to variation in sputum processing protocols, including stringency of sputum decontamination procedures.

Cross-contamination between successively spotted samples is a potentially severe problem and was found to be minimized by increasing the stringency of pin washing procedures beyond the manufacturer's recommendations by increasing sonication in the washing solution for 5 seconds at the highest power setting.

The ethics review procedure and its stringency should be commensurate with the type of research based on an approximate estimation of potential harm.

For every procedure, the maximum stringency filters were utilized to minimize the noise and background results, thereby guaranteeing the fewest number of false-positive results.