The stringency of the chosen conditions was tested on the training set and gradually decreased until it included all proteins in the training set.
Sense negative control hybridizations showed no specific label, demonstrating the high stringency of the hybridization conditions and suggesting high specificity of the riboprobes to the zebra finch transcripts.
Stringent filtering conditions of the combined data sets reduced the number of transcripts from 612 to 259; however, altering the stringency of the filtering conditions and the number of transcripts used did not significantly change the clustering pattern of the individual samples (data not shown).
Outcomes of experiments depend not only on experimental design but also on the stringency of conditions of the experiments: for example, too stringent conditions can lead to false negative interactions, as opposed to false positive results obtained from experiments that were not stringent enough.
Despite gradually increasing the stringency of selection conditions, the selected aptamers did not recognize Aβ40 oligomers but reacted with fibrils of Aβ40, Aβ42, and several other amyloidogenic proteins.
Despite gradually increasing the stringency of selection conditions, the selected aptamers did not recognize A β40 oligomers but reacted with fibrils of A β40, A β42, and several other amyloidogenic proteins.
Strategies that are commonly used to improve the method include increasing the stringency of the co-precipitation conditions and biochemical cross-linking prior to tissue solubilization.
The stringency of our conditions may bias the data set toward conserved repeat elements.
It also indicates that little nonspecific DNA is contaminating the P2 DNA fraction, implying that the stringency of washing conditions in Step 4 is sufficient.
In classical hybridization experiments, cross-hybridizing sequences can be distinguished from identical sequences by increasing the stringency of hybridization conditions.
Three to four selection cycles were conducted, and the stringency of washing conditions was increased every panning round to decrease nonspecific binding.
