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This is attributed to the inclusion of poly I∶C in the EMSA binding reaction to increase the stringency of the assay.
In order to increase the stringency of the assay, a case was considered positive for MV, if at least two of the NP and/or HA antibodies mentioned above were positive.
Because wild-type C. elegans N2 is not susceptible to ZTDO-induced paralysis at 0.5% ZTDO that P. pacificus is, we increased the stringency of the assay by using 10% ZTDO, incubating for 16 hr, and exposing the animals in the absence of OP50 food.
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The ability to control the duration of an enzymatic assay is key to controlling the stringency and hence selection pressure of the assay, and it is an important issue to consider in any directed evolution experiment.
To increase the stringency of assay evaluations, specimens with substantial numbers of polymorphisms in primer binding sites were included.
Its technical implementation by heat inactivation permits reactions to be stopped at any desired time point, permitting variation of assay duration, and hence stringency of the subsequent selection, to be altered at will.
The stringency of the variance allowed for clustering is customizable, thereby affecting the number of peaks that are clustered.
What's more, the stringency of the rules governing each of these dimensions can vary all over the map.
Nevertheless, the framework of parliamentary democracy survived the continuing economic stringency of the 1980s.
The stringency of the FFP punishments gave the regime an authority backed by European law.
Surgical outcomes typically were not related to stringency of the hospital credentialing environment.
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