Sentence examples for stringency of screening from inspiring English sources

We have devised a new strategy for performing directed evolution experiments (Fig. 6A) by increasing stringency of screening while enriching the population in sequences with a desired phenotype, roughly analogous to increased stringency/cycle frequently used in display experiments for selecting high affinity binders [20], [37] [39].

Another strategy to identify minor QTLs is to increase the stringency of phenotypic screening.

This was probably due to differences in the stringency of PQS screening as well as in length of target promoter region.

Differences in stringency of data screening parameters, the taxonomic composition of databases used for the comparative analysis, and methods of phylogenetic analyses can significantly affect the outcome of the study.

We then used a combination of FDR, P value and fold change to adjust the stringency of the screen, with specific criteria described for each example in the text.

In order to increase the stringency of the screens, various concentrations of 3-aminotriazol (3-AT) were used in all the Y1H experiments (from 15 to 60 mM).

For negative controls, ;;UAS-unfF1 virgins were routinely crossed to ;;;OK107-GAL4/+ or ;FRTG13UAS-mCD8/+;;OK107-GAL4/+ males at 22° to continuously monitor and ensure the stringency of the screen.

A fourth argument is that atresia screens oocyte quality and menopause is simply a byproduct of this process, where the age at onset is determined by the stringency of the screen that has been selected for (Stearns and Ebert 2001).

Increasing the stringency of the screen to require a robust raw expression level of 500, P < 0.05 and FC > 2 identified only five genes with elevated expression and 22 genes with reduced expression in mutant podocytes (Fig.  3).

However, if we lowered the stringency of the screen to include hits 2.5 SD above the plate average (Additional File 19), we could identify Snq2p, Cch1p, Mid1p, Pho89p or Fen2p as possible uptake routes.

Unfortunately, a majority of those positive clones from library screening had been eliminated due to less than maximal extension, redundancy, or more likely being false positive as a result of repetitive elements or the initial low stringency library screening.