This indicates that enforcement of a higher-stringency limit on acceptable confidence probability scores generally leads to an increased proportion of unresolved genotypes per dataset.
Any in silico protocol for genome wide GPCR identification should therefore cast a broad enough net to reveal any such highly-diverged receptor groupings, while also providing stringency to limit false positives.
On the other hand, an RCT concerning physician behavior imposes stringencies that limit generalizability.
The inclusion of both edgeR and cufflinks allowed us to detect differentially expressed genes with high stringency while limiting false positives and characterize them using gene ontology enrichment analyses.
Moreover, peptides are considered unique by Scaffold if they differ by as little as one amino acid, and this stringency could also limit the number of unique peptides we report as being common to both peptidomes.
Prior to commencing experiments on fcDNA, we evaluated the performance of the methylation enrichment protocol with low (100 ng) DNA sample amounts, and developed a modified high-stringency protocol to limit the amount of non-specific DNA binding.
Considering that many of the genes we previously studied demonstrating phenotypic changes with allelic ratios of approximately 1.5 to 2-fold would not meet this depth requirement, this level of stringency is severely limiting but necessary if RNA-Seq is used as the sole determinant of AEI.
Many widely used transfection reagents, such as PEI, are polydisperse and often ill-defined (notwithstanding their clear utility in everyday use), which may limit the stringency of any rules even from systematic studies.
Detection of small deletions is theoretically possible with reduced stringencies, but is limited by probe coverage of the gene models and deletion size.
Also, he and airline officials said, there is a limit to the stringency of security measures that can be practically applied to a busy airport.
The stringency was increased thereafter to limit the number of unique reads to four.
