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After all the years of defensive stringency, it is incongruous to witness so brittle a line-up.
There is great and increasing demand for our work for young people with complex disabilities, but in these times of financial stringency it can be difficult for us to get to all the audiences who want us.
Because the isolation index is a measure of stringency, it is particularly attractive for applications in which there is little or no prior biological knowledge to guide the selection of tuning parameters.
Although there seems little doubt that examiners do differ in their stringency, it is much less clear where those differences come from.
At this FDR-level stringency it is difficult to compare the two methods, so we used the ROC AUC (Fig. 7) and Wilcoxon-Mann-Whitney tests to compare their performance at predicting the 11 validated pairs.
In addition to selection stringency, it is also important to co-localize the GOI with the selection marker, for efficient selection and successful amplification of the GOI gene [ 3, 36].
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SM-4 increased stringency further; it required achieving a mean shape value of <0.3 (smaller is preferred).
SM-5 increased stringency further; it required that SM-4 be achieved and that mean, simulated ALC diameters be within 10% of in vitro values.
When applying certain stringency thresholds, it also significantly outperforms PSI-BLAST and BLAST at nearly all of the thresholds (Table 2).
However, these decisions always erred on the side of stringency and it is likely that we have underestimated the total number of ITS-types detected in this study.
Examination scores were adjusted for examiner stringency and it was shown that for the present pass mark, the outcome for 95.9% of candidates would be unchanged using adjusted marks, whereas 2.6% of candidates would have passed, even though they had failed on the basis of raw marks, and 1.5% of candidates would have failed, despite passing on the basis of raw marks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com