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Britain and the Rothschild bank, who were underwriting the Greek loans, insisted on financial stringency from Armansperg.
Increasing the stringency from 2 to 6 peptides resulted in 1504 identified proteins composed of 129 core proteins (8.6%).
HMM searches were performed, as previously described, in a series of threshold E values, increasing stringency from E value < 0.5 to E value < 0.05 (data not shown).
With the decrease of E-value stringency from 0 to lower than 10-6 and 10-3, the BlastX hits number for each transcript increased and the percentage of total contigs matching reference sequences increased (Table 2).
Users may start with this threshold and decide that the results returned are missing a known TFBS, and so on the next analyses, they might reduce the TFBS stringency from 85 to 80%.
In order to identify an optimal consensus level for indel identification, indels were extracted from the 299 alignments under increasing levels of stringency from 25% (the general minimum level observed for homologous proteins or "twilight zone" [ 42]) to 100%, in incremental steps of 5%.
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Second, contigs were further connected through a series of automated merges utilizing consecutive stepping-down stringencies from 1e-50 to 1e-35.
The property of evolving fast implies that such transcripts will disanneal at low stringencies from the heteroduplexes formed by homologous complementary sequences of the two species.
Single isolated colonies from each plate were picked, rigorously vortexed in 500 µl low stringency medium, from which 5 µl drops were placed onto low (SD/−2), medium (SD/−3) or high (SD/−4) stringency selection plates and incubated 3 days at 30°C.
When the filtering stringency increased from F0 to F4, the number of SNPs surviving selection in silico decreased abruptly.
The membranes were hybridised at 64°C using Ambion's Ultrahyb Ultrasensitive hybridisation buffer and washed with Northern Max Low and High Stringency Buffers from Ambion.
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