Sentence examples for stringency controls from inspiring English sources

The negative, positive and stringency controls printed on the array gave no indications for unspecific hybridizations.

Controls included Housekeeping controls for intactness of the biological specimens, negative controls to establish gene expression detection limits and hybridization controls including low and high stringency controls and biotin signal generation controls.

Built-in controls, both sample independent (including staining controls, extension controls, target removal controls and hybridization controls) and sample dependent (including stringency controls, nonspecific binding controls and nonpolymorphic controls), were inspected to assess the quality of the experiment.

The choice of stringency control strategies depends on various factors, including the sensing platform used.

Thermodynamic parameters obtained using the web server DINAMelt allowed supposing the possibility for realization (a) stringency control based on the ionic strength of the hybridization buffer and (b) stringency control based on the temperature elevation.

In this study, we applied two stringency control strategies for surface plasmon resonance (SPR) detection of DNA hybridization and discrimination of completely and partially complementary 24-mer sequences.

For implementation of the second stringency control strategy, SPR spectrometer measuring flow cell with built-in high-precision temperature control and regulation as well as corresponding software was created.

We report the results of the application of two stringency control strategies for SPR detection of DNA hybridization and discrimination of completely and partially complementary oligonucleotides related to the human normal bcr and hybrid bcr-abl genes.

Unlike hybridization suppressors, stringency control based on the ionic strength of the hybridization buffer, which strongly influences hybridization of completely complementary and partially complementary DNA duplexes and does not lead to excessive strong refractive index change, appears quite attractive [10].

The aim of this research was to detect and discriminate the oligonucleotides related to the bcr-abl hybrid mRNA from those related to the normal bcr gene using two stringency control strategies based on the decrease in ionic strength of the hybridization buffer and the temperature elevation by SPR spectrometry biosensor.

In general, we believe the obtained results (especially those obtained due to the stringency control based on the temperature elevation) are very important for the future development of the SPR biosensor for clinical detection of the bcr-abl gene related to some types of leukemia.