Sentence examples for stringency control from inspiring English sources

The choice of stringency control strategies depends on various factors, including the sensing platform used.

Thermodynamic parameters obtained using the web server DINAMelt allowed supposing the possibility for realization (a) stringency control based on the ionic strength of the hybridization buffer and (b) stringency control based on the temperature elevation.

In this study, we applied two stringency control strategies for surface plasmon resonance (SPR) detection of DNA hybridization and discrimination of completely and partially complementary 24-mer sequences.

We report the results of the application of two stringency control strategies for SPR detection of DNA hybridization and discrimination of completely and partially complementary oligonucleotides related to the human normal bcr and hybrid bcr-abl genes.

For implementation of the second stringency control strategy, SPR spectrometer measuring flow cell with built-in high-precision temperature control and regulation as well as corresponding software was created.

Unlike hybridization suppressors, stringency control based on the ionic strength of the hybridization buffer, which strongly influences hybridization of completely complementary and partially complementary DNA duplexes and does not lead to excessive strong refractive index change, appears quite attractive [10].

The negative, positive and stringency controls printed on the array gave no indications for unspecific hybridizations.

Built-in controls, both sample independent (including staining controls, extension controls, target removal controls and hybridization controls) and sample dependent (including stringency controls, nonspecific binding controls and nonpolymorphic controls), were inspected to assess the quality of the experiment.

Controls included Housekeeping controls for intactness of the biological specimens, negative controls to establish gene expression detection limits and hybridization controls including low and high stringency controls and biotin signal generation controls.

A measure of transmissibility and of the stringency of control policies necessary to stop an epidemic is the basic reproductive number, which is the number of secondary cases produced by each primary case [30].

Increasing stringency by controlling primers for cDNA synthesis, designing intron-spanning primers for amplification and the use of alternative validated reference genes during the analysis appears to reduce the number of significant genes at 24 h.