Suggestions(1)
Exact(3)
Solution 3 (high stringency) contained 0.1× SSC.
Solution 2 (medium stringency) contained 0.1× SSC and 0.1% SDS.
Solution 1 (low stringency) contained 2× SSC and 0.1% SDS and was heated to 55°C prior to washing the slides.
Similar(57)
The MiSeq replicates demonstrated a similar tendency as that of the PGM; in general, the lower stringency mapping contained matched reads of short length (avg. 66 bases).
The low stringency (LS) gene set contained probes with a p < 0.05 and a FC > 1.4, while the high stringency gene set (HS) contained probes with a p < 0.01 and FC > 1.5.
Separate cultures of HeLa S3 cells SILAC labeled either with L-arginine and L-lysine (light) or L-arginine-13C6 and L-lysine-13C6-15N2 (heavy) were lysed in ice-chilled ModRIPA buffer (low stringency buffer LS) containing 1% NP-40, 0.1% Na deoxycholate, 150 mM NaCl, 1 mM EDTA, 50 mM Tris, pH 7.5, and protease inhibitors (CompleteTM tablets, Roche Applied Science, Indianapolis, IN).
This suggests that the entire shared single copy genes list is not composed of a random set of genes, with 959 genes shared between Arabidopsis, Populus, Vitis and Oryza (APVO) as defined by the number of PlantTribes [ 63] at stringency 3.0 that contain a single member from each respective genome.
Raw, low stringency selection data for both cell lines contained 10,308 and 8,586 GeneIDs for the Affymetrix and MPSS datasets respectively.
Furthermore, interestingly, we correctly identify the stage of symbiosis for 92%–100% of the proteins contained in modules depending on stringency (see methods and Fig. 3c).
We were able to identify correct annotations for 87%–100% of the proteins contained in modules depending on the stringency of COGs category assignments (see methods and Fig. 3c).
The Apodemus and Rattus lanes contained no distinguishable bands at higher stringency, as was the case when probed with M. m. domesticus probes.
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