Sentence examples for stringency condition from inspiring English sources

Figure 3 demonstrates obvious advantages of the use of a higher stringency condition (0.5 × SSC): depending on the target concentration, the values of the ratio of the sensor response at the P1/mod-Ph hybridization to the sensor response at the Bcrex14/mod-Ph hybridization varied from 1.3 to 1.8 in 2 × SSC and from 2.0 to 2.9 in 0.5 × SSC.

Because the moderate stringency condition (T = 83 °C, Mc = 285 g/kg and Rt = 7 s) completely eliminated detectable S. typhimurium in the test feed matrix, it would appear that all S. typhimurium cells and all mesophilic organisms of similar thermal tolerance would be eliminated at most extruder conditions within the central composite design region.

The development of DNA microarray assays is hampered by two important aspects: processing of the microarrays is done under a single stringency condition, and characteristics such as melting temperature are difficult to predict for immobilized probes.

Utilizing the MSAW to apply the optimal stringency condition for each pair in the Tm matched probe set (method ii) showed that only two of the probe pairs for the nine mutations in the beta-globin gene functioned optimally at a sodium concentration of 17 mM (Figs. 4 and 5B).

The microarrays were washed to highest stringency condition (two 20-min washes in 0.1 × saline sodium citrate and 0.1% sodium docecyl sulfate).

Without enforcing the stringency condition (iii), 12% of the sequences (15% of the sequences present in the network without the singletons) were diagnosed as composite.

Overall, engineering arginines and phenylalanines for increased pKa and hydrogen bonding improved enzyme catalytic efficiency at high stringency conditions.

For in situ application, optimal stringency conditions were adjusted by performing whole cell hybridization using target and nontarget organisms.

Specific amplification and validation of the primers was done by NCBI Primer BLAST (http://www.ncbi.nlm.nih.gov/tools/primer-blast/) against Oryza taxon and high stringency conditions during PCR amplification.

In the chosen low stringency conditions complex patterns were obtained, with a sharp band of ∼700 bp in cases where the vanA gene was present.

For example, Hashimoto and colleagues isolated it as a PACAP receptor from a rat brain cDNA library by cross-hybridization with rat VIP receptor cDNA under low stringency conditions [21].