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High stringency class comparison between CPT-11+PHY906 and PBS treated animals (t test cutoff p-value < 0.001) identified 798 differentially expressed transcripts.
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Whilst this is not an exact science, care was taken where possible to follow a similar selection strategy for each hapten class (increasing stringency though reduction in antigen concentration and the swapping of protein conjugates to minimize the selection of carrier protein specific clones etc).
It's not a sufficient condition; one also has to look at what the stringency is, of course.
At this level of stringency, Bvu0220 is not associated with either Class I or Class II enzymes.
In this technique, a sequential classifier is proposed wherein the classes which require stringent boundaries are tested one by one and once the tests for these classes fail, the stringency of the classifier is increasingly relaxed.
This high stringency selection revealed that the C class included metastases that frequently but not exclusively clustered closer to the A class.
An Atlantic salmon BAC library [ 27] screened at low stringency with radioactive labeled probes for MHC class IIα (DAA) and class IIβ (DAB) hybridized to 20 BAC clones (Table 1).
When we examined all TFBS motif hits (not just the conserved hits) upon all pre-miRs vs. their permuted counterparts, all of the 27 different motif classes were over-represented at high stringency (z ≥ 2.33) among pre-miRs that are not primate-specific, and 24 out of the 27 motif classes were significantly over-represented upon primate-specific pre-miRs.
At this stringency level, cog3618 is subdivided into two primary groups, arbitrarily designated as Class I and Class II.
His win this week involves a very American cry of pain by the country's middle class in the face of globalisation and financial stringency.
The aim of this study was to analyse the MHC class II situation in Atlantic salmon through low stringency screening of available BAC libraries using classical DAA and DAB probes.
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