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Rouhani had presented his cabinet wish-list to parliament on the day he was sworn in, 4 August, in order to show his determination to solve Iran's many problems amid financial stringency at home and international crisis abroad.
The membrane was subjected to a series of stringency washes (high stringency at 56°C) to eliminate nonspecific probe binding.
Stringency at the distal position of the polymer was significantly less than at the central and proximal positions which explains the more solution-like properties of probes placed on longer spacers [13], [30] [33].
The BESs with multiple hits (more than eight) at high stringency (at least 100 bp aligned contiguously) were identified as repeat sequences.
Probes were hybridized overnight under conditions of high stringency at 65°C and detected by using chemiluminescence as recommended by the manufacturer.
However, at least some of the difference is likely attributable to the stringency at which we set our thresholds for DNAm.
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Filters have been provided on the Web site that can adjust the level of BLAST homology, allowing researchers examine stringencies at a level of homology they are interested in.
In addition, we have examined the physical map assembly with extremely high stringencies at p = 10-40, the associated genes from the same BAC contigs still had hits to genes on both chromosome 2 and chromosome 7 in zebrafish.
At the low stringency threshold at which inter-group relationships can be visualized using networks, many of the displayed edges represent poor alignments.
The default filters out homozygous genotype calls if the phred-based score of a heterozygous call is less than 20, medium stringency filters at less than 125, and high stringency filters at less than 200.
Probe hybridisation was conducted overnight at 37°C in a humidified chamber followed by stringency washes at 43°C using 0.1 0.5×SSC plus 30% formamide to remove non-specifically bound probe.
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