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After screening with several rounds of increasing stringency, as is usual with phage technology, the library got overwhelmed by phagemids encoding incomplete scFvs.
The Apodemus and Rattus lanes contained no distinguishable bands at higher stringency, as was the case when probed with M. m. domesticus probes.
Liquid cultures were then grown overnight, aliquoted into 96-well plates, and pin stamped onto synthetic complete media either without uracil (high stringency) or without histidine and with increasing concentrations of 3-AT (Sigma) from 10 to 100 mM (low to high stringency), as well as selection for plasmid maintenance.
He says climate change scientists should adopt the same level of statistical stringency as exists in medicine.
"Rebellion grows in Europe against Merkel's budget austerity," the Spanish daily El País proclaimed on its front page on Thursday, singling out Angela Merkel, the German chancellor, for promoting fiscal stringency as the Continent's only weapon to fight the economic crisis.
Tumblr does not have the same legal stringency as a court – obviously.
Similar(40)
Around 22% of the 454 EST data (66,358 reads) had no significant matches to known or predicted C. elegans transcripts at the specified stringency and as such may represent previously unidentified genetic structures, such as novel transcripts, L1 stage-specific transcripts, novel splice variants and ncRNAs.
As expected, no reads mapped at the highest level of stringency tested, as the requirement for 100% identity would preclude any matches.
Moreover, we also did TBLASTN searches of frog EST data against the Xenopus Genome EST databases with a stringency set as E ≤ 0.0001 and an identity of 90% or higher as candidacy.
The transformants were marked and transferred to higher stringency plates as described earlier.
Probe length, probe spacer length, and assay stringency sufficed as variable parameters in the search for a functional multiplex assay.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com