We therefore employed a higher-stringency approach at the cost of missing more distantly related homologs.
> -wrap-foot> A database of A. thaliana coding sequences organized in TARs was generated for the TAIR annotation v10 as described previously (Rizzon et al. 2006), using a low-stringency approach with a number of N = 10 allowed gene pacers.
In this paper, we used a high stringency statistical approach to identify and characterize 322 ancient non-coding elements (Additional file 2: Table S4) which have remained conserved over large evolutionary distances.
Notably, these cells show partial resistance to loss of anchorage, and the selection can be of limited stringency, compromising approaches based on clonal selection or anyway requiring high stringency.
Using different pathway-level stringencies and approaches, such as Gene Set Enrichment Analysis, PANTHER and Metacore for confirmation of pathway signatures, may increase the robustness of the results.
The stringency of our approach is expected to offer an advantage in a variety of applications.
We found that the resulting stringency of this approach is not adequate for the analysis of published gene expression signatures [35].
The stringency of this approach, in combination with genome-wide genotypes from the same individuals, is anticipated to enable a genomic convergence analysis that advances understanding of the molecular basis of SCZ.
Finally, the rigorous statistical analysis employed in the development of the PA underlines the stringency of our approach.
