Exact(12)
For our 'low stringency' analysis, up- and downregulated genes were determined by using Wilcoxon's signed rank test to compare mutant and control microarrays in a pairwise fashion with a p-value = 0.002.
For our 'low stringency' analysis, up- and downregulated genes were determined as described above for the single mutants with the exception that we required that a given feature be up- or downregulated in all six pairwise comparisons in order to be included in our low stringency dataset.
In this paper we address these issues, and propose a new methodology for the analysis of micro-array transcriptional data in which the stringency analysis threshold is not only determined using statistical approaches but also intertwined with biological considerations to allow for a more specific and sensitive selection of the differentially regulated genes.
Using FDR corrected analysis the results are less evident but consistent with the low stringency analysis.
Nemhauser et al. defined marker genes as those genes specifically regulated by one hormone in a high stringency analysis and not by any other hormone even in a lower stringency analysis [ 22].
Gene lists produced by the initial low stringency analysis were considered to be the transcriptome of each respective cell line.
Similar(48)
The high-stringency analysis from this previous work provided a base definition of duplication status for this analysis.
At higher stringency, our analysis may be more comprehensive.
One allele (C*17:01 01 from HIV_114) was only detected with the lower stringency, modified analysis pipeline described above.
To achieve a high stringency of analysis, we required independent prediction of positive or negative selection by both methods.
The optimal coverage thresholds also depend on other factors, such as the suspected type of mutation, whether somatic or inherited, and the stringency of analysis.
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