Sentence examples for stringency according to the from inspiring English sources
Replica plating was performed under conditions of increasing stringency according to the manufacturer's suggestions and subsequently analysed for growth on –Leu/-Trp/-His, -Leu/-Trp/-His/-Ade synthetic drop out (SD) media.
DIG-labeled probe was hybridized in DIG Easy Hyb buffer (Roche) overnight at 50°C and washed at moderate stringency according to the manufacturer's instructions.
Hybridizations were performed at 42°C and the blots were washed at high stringency according to the manufacturer's instructions, and then exposed in a PhosphorImager cassette for one week.
Microarrays were washed using buffers of increasing stringency according to the manufacturer's instructions, scanned with a ScanArray 5000 Microarray Analysis System (PerkinElmer Life Sciences Inc ,Boston, MA) and the images were quantified using Microarray Imager software (CombiMatrix).
Hybridisation was performed overnight at 65°C and the blot washed in high stringency according to instructions given by the manufacturer.
Probe labeling using the DIG DNA labeling Kit (Roche, Applied Science) and hybridization were performed under high stringency conditions, according to the manufacturer's instructions.
The custom flexibility of the array platform facilitated interrogation of loci at differing stringencies, according to a gene prioritization strategy that allows saturation of high priority loci with a greater density of markers than the existing GWAS tools, particularly in African HapMap samples.
The membrane was washed using the Ambion Northern Max Low Stringency and High Stringency wash solutions according to the manufacturer's instructions.
Stringency washes were performed according to the Rapid-hyb protocol (Amersham).
Labelled samples were hybridised to BeadChips overnight (18 hours) at 55°C and stringency washes were performed according to the manufacturer's protocol.
The results are ordered according to the stringency of the method (from OrthoMCL90 > OrthoMCL80 > OrthoMCL70 > OrthoMCL60 > blastp > HMMscan > tblastn).
