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Rather, the HMW and LMW fragments indicate that nucleases act on DNA that is still protected by chromatin, with HMW fragments presumably reflecting nuclease accessibility of higher-order chromatin organization (e.g., loops; see [4]) and LMW fragments that of a nucleosomal, beads on a string organization.
Therefore, a comparative genomic analysis was performed using the M. flocculare contigs (MFL contigs) and M. hyopneumoniae genome, considering the ORF string organization and OC distribution (Additional file 7A-B).
The inverted segment present in the 232 strain was further analysed and compared with the cognate 7448 region, taking into consideration the ORF string organization and OC distribution (Fig. 3).
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