This revealed a striking, bimodal binding pattern, with dispersed ORC1/CDC6 binding in the interior of chromosomes and denser binding at subtelomeres.
It is particularly striking that binding sites for known RAS pathway activators are associated both with activating EGFR mutations and with erlotinib sensitivity.
In striking contrast, the binding of Bmi1 was not restored at all and that of Ring1B was only mildly affected (Figure 4B and C).
Despite these similarities in early RNP assembly, there were striking differences in binding of different transcript classes to cytoplasmic and nuclear surveillance factors.
However, it should also be noted that the murine and human GAD65 proteins used in our experiments were expressed in insect cells and wheat germ lysates, respectively, so we cannot rule out that some difference in post-translational modifications could also explain the striking difference in binding to NOD autoantibodies.
Furthermore, when comparing each EWS-FLI1-correlated cluster to the corresponding EWS-FLI1-anticorrelated cluster, we observed a striking enrichment for binding of E2F transcription factors among the EWS-FLI1-correlated transcripts, reinforcing a previously reported link between EWS-FLI1 and E2F (Bilke et al., 2013).
We hypothesized that these non-conserved residues in the PA-binding interface are responsible for the striking differences in PA binding affinity, receptor-specific pH thresholds for pore formation, and even translocation of the toxin.
Despite striking functional similarities, radioligand binding studies revealed different mGluR5 binding profiles for DFB and CDPPB compared with those of CPPHA (19, 21, 27).
Comparison of the recently solved high-resolution structures of the sodium-translocating bacterial rhodopsin and various Na+-binding GPCRs revealed striking similarity of their sodium-binding sites.
The bill would bar public employees from striking and would prohibit binding arbitration for police officers and firefighters.
