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A stricter cut-off value of 0.9 was used in this study.
Other studies have used a similar or stricter cut-off.
We use a stricter cut-off for research purposes (8.0 mmol∙l−1).
We also tested the impact of applying alternative (stricter) cut-offs; these did not affect our general findings.
In this study, another parameter σ, which amplifies the significance level by a factor of (1 + σ), was proposed to create a stricter cut-off.
In these cases, a stricter cut-off value for deciding the presence of a gene (5.6-5.7 instead of 5.5) is used.
We also evaluated a stricter cut-off of 20 points or more as used for the OARSI responder criteria, as opposed to minimal clinically perceptible change [ 22].
The stated conclusion was that "there is a great variability among anti-dsDNA assays and a stricter cut-off limit must be applied to acceptable SLE specificities of FIDIS, ELiA and EUROLINE".
Furthermore, the prevalence of MS reported in this study - around 20% - might stem from the employed definition, which relied on stricter cut-off points than those used in other studies [ 11].
Using a stricter cut-point of 400 IU/d, the Montreal researchers reported that 74% of exclusively breastfed infants and 51% receiving mixed feeds achieved an intake of 400 IU/d at 6 months [ 18].
A cut-off of 20% in our data categorises a hub as any protein with a degree ≥3, we therefore chose a stricter cut-off of 2% so a hub is only classified as such with a degree ≥23.
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