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Innate lymphoid cells (ILCs), characterized by their strict tissue residence and distinct function in the mucosa, are attracting increased attention in innate immunity.
So how are normal cells, which are part of a strict tissue organization with cellular social cues, transformed into cellular sociopaths who disregard tissue order and ignore cellular dialog?
This data indicates that CDCP genes exhibit a strict tissue specific expression which is also developmentally controlled.
Thus proper stage of tissue collection, strict tissue storage conditions, efficient extraction and purification steps to obtain material free of endogenous host cell proteins/components are critical in achieving desired efficacy and safety of the therapeutic.
The salmon genome contains homologs of mammalian contractile proteins that are expressed in smooth muscle and non-muscle cells but strict tissue specificity was most probably acquired after the evolutionary separation of the teleost and tetrapod lineages.
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The required functions may depend on the micro-environmental conditions, cell type, or subcellular localization, and the differential expression of regulatory miRNAs may facilitate the process for obtaining strict tissue-specific and timely control of such functions.
Another example is the fact that miRNAs are often expressed in a strict tissue-specific manner during development; for instance, miR-124a is expressed only in brain and spinal cord of vertebrates and ventral nerve cord of insects [40], [41], [42].
The peptide encoding gene shows a strict tissue-specific and developmentally regulated expression pattern.
However, notably due to their strict tissue-specific expressions, mJRP-32 genes are not supposed to be orthologous, although the proteins share numerous common features [ 82].
Nevertheless, this partial or complete functional redundancy is expected to require strict tissue-specific regulation in order to prevent the damage that an excessive level of Brachyury protein could cause.
The expression pattern of individual miRs with strict tissues, the clinical-feature-specificity or the different target genes involved in the unique regulation network of EHCC may all involved in the effect of miR-34a on Smad4 [ 35, 45].
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Justyna Jupowicz-Kozak
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