Platypus is an exception however, being the only animal found to have conserved Gly193 in KLK10, probably indicating that this mutation was later introduced in order for the protein to acquire a very strict specificity and likely a highly specific biological function.
After overexpression in Saccharomyces cerevisiae, VfCBR1A was enzymatically active, and like its Arabidopsis ortholog, displayed strict specificity for NADH as the reductant.
Upon cytidine binding, a large induced fit was observed at the uridine/cytidine binding site, which endows UCK with a strict specificity for pyrimidine ribonucleosides.
Inhibitors that show strict specificity for one protease usually achieve this selectivity by combining substrate-like binding in the active site with exosite binding on the protease surface.
Protein extraction using sodium dodecyl sulfate and 2-mercaptoethanol ensured strict specificity of the ELISA, and components of three popular processed foods had no effect on the ELISA response.
The 1.7 Å crystal structure of the engineered variant, iodoTyrRS-mj, bound with 3-iodo-l-tyrosine revealed the structural basis underlying the strict specificity for this nonnatural substrate; the iodine moiety makes van der Waals contacts with 5 residues at the binding pocket.
The strict specificity of the designed primers and selected probes for hazelnut was demonstrated by performing PCR amplification of genomic DNA extracted from different hazelnut varieties and other species of similar families (pistachio, cashew, walnut and tangerine) and analyzing the resultant amplicons by the developed electrochemical sensor.
Given the strict specificity of IRBC C4S interactions, it is better to use either placental CSPG or CSPGs bearing structurally similar C4S chains in defining VAR2CSA structural architecture that interact with C4S, evaluating VAR2CSA constructs for vaccine development or studying structure-based inhibitors as therapeutics for PAM.
Alliinase shows a strict specificity toward alliin except that onion root isoforms, which are different in glycosylation, exhibit an additional activity toward L-cystine to a limited extent (Lancaster et al. 2000).
