The gfp fragment under control of a strict low-iron-regulated promoter Pviua was inserted into seven plasmids with varied replicons derived from pAT153, pACYC184, pBBR1, pEC, pMW118, pRK2, and pSC101, to generate seven corresponding plasmids.
The leaders were rebuffed, a startling act of insubordination for a group that prides itself on strict hierarchy and iron discipline.
Germany is quite an amazing country, where unlimited freedom to work and explore new directions meets strict order and iron discipline, where unlimited resources come up against strict planning and careful pragmatism, and where politeness and courtesy are mixed with a special sense of humour and generosity.
However, life in the presence of oxygen requires a strict regulation of iron metabolism in all organisms.
Thereinto, PfhuA1, which could be strict repressed by excess iron and efficiently induced by iron chelators, was the ideal promoter candidate for toxic gene expression in E. coli host.
In a concurring opinion, Justice Thomas insisted unapologetically that, because teachers in the colonial era imposed strict discipline "with an iron hand," students today should have no First Amendment rights of freedom of speech in public schools.
The present study employed stricter criteria to define iron status.
Only boys complied and benefited from iron distributed with strict supervision (third study).
Iron Maiden had a strict and organised routine that suited the band's writing style, which Dickinson described as a "time table".
Thus, iron acquisition is under strict regulation to maintain a balance of intracellular concentration.
