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To analyze only live cells, a strict gate was set in forward- and side-scatter dot plot including the live population.
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Using the strict gating parameters reported recently [8], Treg were identified as CD4 positive cells with brighter CD25 staining than that of the CD4-negative population (Fig 1a).
Additionally, the heterogeneity was not due to cell size variation, as strict gating on the FSC/SSC was used to limit the analysis to cells of uniform size/physical characteristics (<10% of total population) (Figure S6).
With no strict gate-keeping system, patients can easily access secondary care in tertiary hospitals, and there are no differences in the benefits provided to beneficiaries in the HI and the MA programs [ 8].
In order to avoid false positive detection, we applied a strict gating strategy (online resource 2).
A strict gating strategy was used to determine different immune cell populations as previously described [ 35].
It further suggests that although some HSCs would be lost, setting stricter gates for CD150 and EPCR expression would enrich for more highly purified fractions.
Highly purified EPCR++CD150+CD48−CD45+ (E-SLAM) HSCs have previously been found to be highly efficient (>90%) at making colonies under supportive cell culture conditions when a strict viability gate is set [12,13].
When flow-sorting data were analyzed, two populations were identified that would typically be excluded by a strict viability gate: a low forward scatter (FSC) population and a 7-AADdim (7-AAD = 7-aminoactinomycin D) population (Fig. 2A).
Similarly, whereas Juul Labs applies strict age-gating when selling its products through its website, many of the accused products appear to be sold with little or no real age-verification processes.
And the rules about who can enter its locked gates seem strict enough to keep it that way for a long time.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com