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We feel that selecting an expression cutoff of ln TPM) = 0 is too strict for this analysis as many imprinted genes expressed below that level, that we carefully validated, have features of known and novel-validated imprinted genes expressed above that level, e.g., located in imprinted clusters, isoform-specific imprinting, temporal and spatial regulation, and associated with apoptosis.
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Discipline was strict for the trainee officers.
"There are very strict numbers for this," the man can be heard saying.
The food and beverage manager, Brett Archer, said the park's fire management office had issued strict instructions for this holiday season.
The 1.7 Å crystal structure of the engineered variant, iodoTyrRS-mj, bound with 3-iodo-l-tyrosine revealed the structural basis underlying the strict specificity for this nonnatural substrate; the iodine moiety makes van der Waals contacts with 5 residues at the binding pocket.
Calvary, a competing Christian school where I would eventually graduate, used the same words, but it wasn't strict enough for this pastor, and he would constantly bad-mouth it as "liberal".
Detection of additional X-linked genes was limited by our strict criteria for this screen (i.e. two sites, both >1.5 SD above the mean).
We had strict criteria for this diagnosis, and when reasonable doubts arose, the case was not included in our series.
In this regard, a low precision value for the "association" is caused by failing to use strict templates for this type of interaction.
Nevertheless, there is no strict definition for this term and some authors include pioneer neurons in this concept (Miller et al. 2010).
Another relevant aspect is that the diagnosis of GDM in this study was performed according to Carpenter Coustan thresholds [ 8] while the most current consensus statement has recommended more strict values for this diagnosis [ 1].
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