The isolation identification method can give qualitative and quantitative results, but it is time-consuming and requires strict experiment conditions and professional experimenters.
It is important to note that many genes identified as expressed by the nCounter analysis system had microarray expression values lower than those identified as unexpressed, confirming on a new platform the observation made by Irizarry and colleagues [ 35, 36] that probe-effects prevent strict, experiment-wide thresholding of expressed genes.
Regarding future works, the current experiment is unbalance in participants' gender and the appearance of different morphing conditions, a stricter experiment with female participants ought to be done in the future.
However, fixing such strict conditions of experiments limits the usage of eye trackers in a laboratory environment.
The procedure includes three design elements that (together) have not been featured in similar experiments: strict ambiguity preferences, a single decision, and a mechanical randomization device with an unknown distribution (to both subjects and experimenters).
They did not, however, perform the strict lineage tracing experiments required to quantitatively assess the relative contribution of CPCs to the adipogenesis in their models.
The combination of a strict design of experiment with a robust detection method, applied on sodium valproate, validated the possibilities of untargeted metabolomics in hepatic toxicological research.
To prevent animal caretakers from acting as a vector of transmission, during the entire experiment strict hygienic measures were used.
After 5 dpf embryos fall under strict local animal experiment regulations, therefore most embryos were not followed for longer periods.
